Journal: The Science of the Total Environment

Article Title: Sampling methods and assays applied in SARS-CoV-2 exposure assessment

doi: 10.1016/j.scitotenv.2021.145903

Figure Lengend Snippet: Data obtained from the chosen articles.

Article Snippet: , 43. Aerosol and surface contamination of SARS-CoV-2 observed in quarantine and isolation care , USA , No , Surface and air samples from COVID-19 patient rooms , Air sampling: Sartorius Airport MD8 air sampler operating at 50 Lpm for 15 min. Surface samples: sterile swabs , Viral RNA Extractions: using a Qiagen DSP Virus Spin Kit. RT-qPCR: using Invitrogen Superscript III Platinum One-Step Quantitative RT-qPCR System. Primers and probe used target the E gene of SARS-CoV-2. , • We detected viral contamination among all samples. , ( ) .

Techniques: Sampling, Lysis, RNA Extraction, Environmental Monitoring, Virus, Multiplex Assay, Northern Blot, Marker, RNA Detection, Isolation, Membrane, Control, Environmental Sampling, Amplification, Transmission Assay, Aerosol, Diagnostic Assay, Infection, Sterility, Real-time Polymerase Chain Reaction, Nested PCR, Reverse Transcription, Extraction, Purification, Digital PCR, Preserving, Quantitative RT-PCR, cDNA Synthesis, Magnetic Beads, Incubation, Modification, One Step RT-PCR, Cell Culture, Sequencing

Journal: bioRxiv

Article Title: Red yeast rice-derived MKA ameliorates cardiac hypertrophy in hypertensive rats by inhibiting ERK1/2/c-Fos pathway

doi: 10.64898/2026.03.10.710945

Figure Lengend Snippet: Representative images of EDU and TUNEL staining ( A ), EDU positive percent ( B ) and apoptosis percent ( C ) in H9c2 cardiomyocytes treated with different concentrations of MKA (1, 5, 10 μM) and 200 nM Ang II for 24 hours. D-H. Representative Western blots and relative protein expression for β-MHC, p-ERK, t-ERK, c-Fos, and Caspase-3. Data are presented as mean ± SE, comparisons among multiple groups were conducted using one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test (n = 4 per group; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). LM: 1 μM MKA; MM: 5 μM MKA; HM: 10 μM MKA;

Article Snippet: Cells were fixed with 4% paraformaldehyde, permeabilized with 0.3% Triton X-100/PBS (VICMED) for 5 minutes, and processed using the One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594) (Elabscience Co., Ltd., Wuhan, E-CK-A322) according to the manufacturer’s instructions.

Techniques: TUNEL Assay, Staining, Western Blot, Expressing, Comparison

Journal: Viruses

Article Title: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

doi: 10.3390/v12060582

Figure Lengend Snippet: Summary table of available protocols posted to the WHO’s website.

Article Snippet: National Institute of Infectious Diseases, Japan , N gene , NR , , , Average Cq value of specimen was 36.7 and 35.0 for the positive control (500 copies of RNA transcript) , , , NR , 1 μL of 20 xprimer and probe mix in a 20 μL reaction with 5 μL of RNA. F primer at 500 nM, R primer at 700 nM, probe at 200 nM. , RNA extracted using QIAamp viral RNA mini kit (Qiagen). Reverse transcription via Super Script IV Reverse Transcriptase (Thermo). RT-PCR via QuantiTect Probe RT-PCR Kit (Qiagen).

Techniques: Amplification, Concentration Assay, RNA Extraction, Virus, Sequencing, Extraction, Diagnostic Assay, Positive Control, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, One Step RT-PCR, Cell Culture